538. DNAvaletTM Transfection Reagent

Transfection of murine dendritic cell line (JAWS II) by a nonviral transfection reagent.

Dendritic cells are the most potent antigen-presenting cells that initiate and modulate the host immune system. Based on their immunostimulatory activity, a variety of strategies have been developed to use dendritic cells as vaccines and immunotherapeutic agents against infection and cancer. Genetically modified dendritic cells are useful for immunotherapeutic purposes because of their sustaine...

A peptidomimetic siRNA transfection reagent for highly effective gene silencing.

RNA interference (RNAi) techniques hold forth great promise for therapeutic silencing of deleterious genes. However, clinical applications of RNAi require the development of safe and efficient methods for intracellular delivery of small interfering RNA (siRNA) oligonucleotides specific to targeted genes. We describe the use of a lipitoid, a cationic oligopeptoid-phospholipid conjugate, for non-...

FuGENE® 6 Transfection Reagent: minimizing reagent-dependent side effects as analyzed by gene- expression profiling and cytotoxicity assays

Transfection protocols and the reagents used to deliver nucleic acids into cells can affect cell physiology, leading to misinterpretation of results. Using microarray analysis and cell viability assays, we evaluated different transfection reagents to determine their effects on gene expression profiles. Results show that FuGENE 6 Transfection Reagent exhibits very low cytotoxicity, delivers high...

Improved transfection technique for adherent cells using a commercial lipid reagent.

Highly Efficient Reverse siRNA Transfection of Human Epithelial Lung Carcinoma A549 Cells Using siLentFect Lipid Reagent

Methods Transfection A549 cells (American Type Culture Collection, ATCC #CCL-185) were grown in F-12K nutrient mixture, Kaighn’s modification (Invitrogen Corporation) with 10% fetal bovine serum. Transfection complexes were prepared in Opti-MEM I reduced-serum medium (Invitrogen). Human GAPDH siRNA and nonsilencing siRNA were used (siLentMer starter kit for human GAPDH, Bio-Rad Laboratories, In...